Baseline correction with trendtool: Difference between revisions

Revision as of 12:42, 3 October 2016

Zoom into peak of interest, in this case the peak at 1060, then select add marker and move the marker to the peak by left clicking and dragging.
Right click the marker, and select area. A second marker will appear.
Right click either of the markers and select add reference. A dashed line will appear.
Right click the reference point, and select Baseline. A second dashed line will appear.
Drag the dashed lines to the desired points to use for two-point baseline correction.
Now take a look at the second figure. Because the dataset consisted of 10 spectra, the second figure plots the area under the curve and above the baseline for each of the 10 spectra in the dataset.

Fig. 2. Effect of varying cost parameter, with gamma = 0.01
a) zoom into peak of interested and select add marker
b) Right click the marker and select area
c) A second marker will appear.
d) select add reference, a dashed line will appear.
e) select add baseline, a second dashed line will appear.
f) drag the dashed lines to the desired points for two-point baseline correction
g) area under the curve and above the baseline for each sample

@@ Line 13: / Line 13: @@
 File:area-2.jpg|b)  ''Right click the marker and select area''
 File:second_marker_2.jpg|c)  ''A second marker will appear.  ''
-File:add_reference_2.jpg|d)  ''select add reference''
+File:add_reference_2.jpg|d)  ''select add reference, a dashed line will appear.''
-File:add_baseline_2.jpg|e)  ''select add baseline''
+File:add_baseline_2.jpg|e)  ''select add baseline, a second dashed line will appear.''
 File:final_2.jpg|f)  ''drag the dashed lines to the desired points for two-point baseline correction''
 File:calc_2.jpg|g)  ''area under the curve and above the baseline for each sample''
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